Characterization of Recombinant, Ureolytic Streptococcus mutans Demonstrates an Inverse Relationship between Dental Plaque Ureolytic Capacity and Cariogenicity

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Characterization of recombinant, ureolytic Streptococcus mutans demonstrates an inverse relationship between dental plaque ureolytic capacity and cariogenicity.

Dental caries results from prolonged plaque acidification that leads to the establishment of a cariogenic microflora and demineralization of the tooth. Urease enzymes of oral bacteria hydrolyze urea to ammonia, which can neutralize plaque acids. To begin to examine the relationship between plaque ureolytic activity and the incidence of dental caries, recombinant, ureolytic strains of Streptococ...

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AIM Streptococcus mutans and Streptococcus sobrinus are the main pathogens associated with the development of dental caries in humans. Recently, the real-time polymerase chain reaction (qPCR-TR) has been used for fast and exact quantification of these bacteria species. This molecular biology method has made the detection of these bacteria in saliva and dental plaque possible; additionally, i...

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Physiological study of an obligately anaerobic ureolytic bacterium.

Urease is an enzyme of wide distribution as evidenced by the fact that some 200 species of bacteria, as well as higher plants and animals have been found to possess it (Sumner and Somers, 1953). Pasteur and Miguel were reported to have isolated "urobacilli" from air, soil, and sewage (Cooke and Keith, 1927). Subsequently, the enzyme was found in many soil and intestinal bacteria (Gibson, 1934; ...

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Cariogenicity features of Streptococcus mutans in presence of rubusoside.

BACKGROUND One promising way of reducing caries is by using sucrose substitutes in food. rubusoside is a prototype sweet substance isolated from the leaves of the plant Rubrus suavissimus S. Lee. (Rosaceae), and is rated sweeter than sucrose. The purpose of this study was to investigate the effects of rubusoside on Streptococcus mutans growth, acidogenicity, and adherence to glass in vitro. M...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 2000

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.68.5.2621-2629.2000